Ag100Pest Initiative

The Ag100Pest Initiative is led by the USDA’s Agricultural Research Service. The initiative leverages ARS's unique expertise in both arthropod pest management and agricultural genomics research, and enhances the agency’s contribution to two international genome sequencing projects – i5K and the Earth BioGenome Project (to sequence the genomes of all of the world’s 1.5 million animals and plants).

Goal: Produce annotated, reference quality genome assemblies for the top 100 US arthropod agricultural pests. When possible, generate the PacBio long-read data from a single individual.

Arthropod pests of US field crops, livestock, bees, trees, rangeland and stored products as well as foreign pest species considered potential invasive threats to US agriculture are included. Beyond genomes, Ag100Pest teams will develop best practices that will benefit the entire arthropod genomics community. We are interested in collaborating with the community in these efforts and hope our successes will encourage others to undertake similar efforts.

Executive Committee: Anna Childers, Brian Scheffler, Kevin Hackett
Core Team: Brad Coates, Scott Geib, Brian Scheffler, Tim Smith, Anna Childers, Monica Poelchau, Chris Childers, Kevin Hackett

The 169 genome sequencing projects in the table below are currently included in the effort. Some species will have more than one assembly where there is value in comparing among sexes, populations, or subspecies. This list is not final; additions and modifications may occur as the project progresses.
Last updated: 2021-03-09

OrderFamilyScientific NameCommon Name
ColeopteraAnobiidaeLasioderma serricorneCigarette beetle
ColeopteraAnobiidaeStegobium paniceumDrugstore beetle
ColeopteraBostrichidaeProstephanus truncatusLarger grain borer
ColeopteraBostrichidaeRhyzopertha dominicaLesser grain borer
ColeopteraBrentidaeCylas formicariusSweet potato weevil
ColeopteraBuprestidaeAgrilus planipennisEmerald ash borer
ColeopteraCerambycidaeAcalolepta aestheticaQueensland longhorned beetle
ColeopteraCerambycidaeAnoplophora chinensisCitrus long-horned beetle
ColeopteraCerambycidaeMallodon dasystomusHardwood stump borer
ColeopteraCerambycidaeTetropium fuscumBrown spruce longhorn beetle
ColeopteraCerambycidaeTrichoferus campestrisVelvet longhorned beetle, Mulberry longhorn beetle
ColeopteraChrysomelidaeAcalymma vittatumStriped Cucumber Beetle
ColeopteraChrysomelidaeAphthona nigriscutisFlea beetle, Leafy spurge beetle
ColeopteraChrysomelidaeChrysomela scriptaCottonwood leaf beetle
ColeopteraChrysomelidaeDiabrotica barberiNorthern corn rootworm
ColeopteraChrysomelidaeDiabrotica cristataBlack Diabrotica
ColeopteraChrysomelidaeDiabrotica undecimpunctataSouthern corn rootworm
ColeopteraChrysomelidaeDiabrotica undecimpunctataSouthern corn rootworm
ColeopteraChrysomelidaeDiabrotica virgifera virgiferaWestern corn rootworm
ColeopteraChrysomelidaeDiorhabda carinataLarger tamarisk beetle
ColeopteraChrysomelidaeDiorhabda carinulataNorthern tamarisk beetle
ColeopteraChrysomelidaeDiorhabda elongataMediterranean tamarisk beetle
ColeopteraChrysomelidaeDiorhabda sublineataSubtropical tamarisk beetle
ColeopteraChrysomelidaeXanthogaleruca luteolaElm leaf beetle
ColeopteraCurculionidaeAnthonomus grandis grandisBoll weevil
ColeopteraCurculionidaeAnthonomus grandis thurberiaeThurberia weevil
ColeopteraCurculionidaeCallosobruchus maculatusCowpea weevil
ColeopteraCurculionidaeCurculio caryaePecan weevil
ColeopteraCurculionidaeDiaprepes abbreviatusCitrus root weevil
ColeopteraCurculionidaeHypera posticaAlfalfa weevil
ColeopteraCurculionidaeHypothenemus hampeiCoffee berry borer
ColeopteraCurculionidaeSitophilus granariusGranary weevil
ColeopteraCurculionidaeSitophilus oryzaeRice weevil
ColeopteraCurculionidaeSitophilus zeamaisMaize weevil
ColeopteraDermestidaeDermestes maculatusCommon hide beetle
ColeopteraDermestidaeTrogoderma granariumKhapra beetle
ColeopteraDermestidaeTrogoderma inclusumLarger cabinet beetle
ColeopteraDermestidaeTrogoderma variabileWarehouse beetle
ColeopteraNitidulidaeAethina tumidaSmall hive beetle
ColeopteraNitidulidaeCarpophilus hemipterusDried fruit beetle
ColeopteraScarabaeidaeOryctes rhinocerosCoconut rhinoceros beetle
ColeopteraSilvanidaeOryzaephilus mercatorMerchant grain beetle
ColeopteraSilvanidaeOryzaephilus surinamensisSaw-toothed grain beetle
ColeopteraTenebrionidaeCynaeus angustusLarger black flour beetle
ColeopteraTenebrionidaeGnatocerus cornutusBroad-horned flour beetle
ColeopteraTenebrionidaeLatheticus oryzaeLongheaded flour beetle
ColeopteraTenebrionidaePalorus ratzeburgiiSmall-eyed flour beetle
ColeopteraTenebrionidaeTenebrio molitorYellow mealworm
ColeopteraTenebrionidaeTribolium brevicornisNorth American flour beetle
ColeopteraTenebrionidaeTribolium castaneumRed flour beetle
ColeopteraTenebrionidaeTribolium castaneumRed flour beetle
ColeopteraTenebrionidaeTribolium confusumConfused flour beetle
ColeopteraTenebrionidaeTribolium destructorDestructive flour beetle
DipteraBibionidaePlecia nearcticaLove bug
DipteraCecidomyiidaeMayetiola avenaeOat stem midge
DipteraCecidomyiidaeMayetiola destructorHessian fly
DipteraCecidomyiidaeMayetiola hordeiBarley stem gall midge
DipteraCecidomyiidaeResseliella maximaSoybean gall midge
DipteraCulicidaeAedes bromeliaemosquito
DipteraCulicidaeAedes liliimosquito
DipteraMuscidaeHaematobia irritansHorn fly
DipteraMuscidaeHaematobia irritansHorn fly
DipteraMuscidaeMusca autumnalisFace fly
DipteraMuscidaeMusca autumnalisFace fly
DipteraMuscidaeStomoxys calcitransStable fly
DipteraMuscidaeStomoxys calcitransStable fly
DipteraTephritidaeAnastrepha fraterculusSouth American fruit fly
DipteraTephritidaeAnastrepha ludensMexican fruit fly
DipteraTephritidaeAnastrepha ludensMexican fruit fly
DipteraTephritidaeAnastrepha obliquaWest Indian Fruit Fly
DipteraTephritidaeAnastrepha suspensaCaribbean fruit fly
DipteraTephritidaeBactrocera albistrigataTephritid fruit fly pest
DipteraTephritidaeBactrocera correctaTephritid fruit fly pest
DipteraTephritidaeBactrocera dorsalisOriental fruit fly
DipteraTephritidaeBactrocera dorsalisOriental fruit fly
DipteraTephritidaeBactrocera zonataTephritid fruit fly pest
DipteraTephritidaeCeratitis capitataMediterranean fruit fly
DipteraTephritidaeRhagoletis completaWalnut husk fly
DipteraTephritidaeStrauzia longitudinalisSunflower maggot
DipteraTephritidaeStrauzia vittigeraSunflower maggot
DipteraUlidiidaeChaetopsis massylaCornsilk fly, Picture-winged fly
DipteraUlidiidaeEuxesta elutaCornsilk fly, Picture-winged fly
DipteraUlidiidaeEuxesta stigmatiasCornsilk fly, Picture-winged fly
HemipteraAclerdidaeNipponaclerda biwakoensisRoseau cane scale
HemipteraAleyrodidaeNAWhitefly
HemipteraAphididaeAphis fabaeBlack bean aphid
HemipteraAphididaeAphis gossypiiCotton aphid
HemipteraAphididaeAphis spiraecolaGreen citrus aphid
HemipteraAphididaeEriosoma lanigerumWoolly apple aphid
HemipteraAphididaeMacrosiphum euphorbiaePotato aphid
HemipteraAphididaeMelanaphis sacchariSugarcane aphid
HemipteraAphididaeSchizaphis graminumGreenbug
HemipteraAphididaeSipha flavaYellow sugarcane aphid
HemipteraCicadellidaeGraphocephala atropunctataBlue-green sharpshooter
HemipteraCicadellidaeHomalodisca vitripennisGlassy-winged sharpshooter
HemipteraCoreidaeAnasa tristisSquash bug
HemipteraCoreidaeLeptoglossus clypealisWestern leaf-footed bug
HemipteraCoreidaeLeptoglossus zonatusLeaf-footed bug
HemipteraDiaspididaeAonidiella aurantiiCalifornia red scale
HemipteraFulgoridaeLycorma delicatulaSpotted lanternfly
HemipteraGeocoridaeGeocoris pallensWestern big-eyed bug
HemipteraLygaeidaeOncopeltus fasciatusLarge milkweed bug
HemipteraMiridaeLygus hesperusLygus bug, Western tarnished plant bug
HemipteraMiridaeLygus lineolarisLygus bug
HemipteraPentatomidaeBagrada hilarisBagrada bug
HemipteraPentatomidaeEuschistus servusBrown stink bug
HemipteraPentatomidaeHalyomorpha halysBrown marmorated stink bug
HemipteraPentatomidaeMurgantia histrionicaHarlequin bug
HemipteraPentatomidaeNezara viridulaSouthern green stink bug
HemipteraPentatomidaePiezodorus guildiniiRed-banded stink bug
HemipteraPlataspidaeMegacopta cribrariaKudzu bug
HemipteraPseudococcidaeFerrisia gilliGill's mealybug
HemipteraPseudococcidaeNAMealybug
HemipteraPseudococcidaePlanococcus ficusVine mealybug
HemipteraPseudococcidaePseudococcus maritimusAmerican grape mealybug
HemipteraPsyllidaeCacopsylla pyricolaPear psylla
HemipteraOxycarenidaeOxycarenus hyalinipennisCotton seed bug
HemipteraScutelleridaeEurygaster integricepsSunn Pest
HemipteraTriozidaeBactericera maculipennisBindweed psyllid
HemipteraTriozidaeLeuronota fagaraeLime psyllid
HymenopteraAphelinidaeAphytis melinusAphytis
HymenopteraBraconidaeMicroplitis mediatorEndoparasitoid
HymenopteraDiprionidaeDiprion similisIntroduced pine sawfly
HymenopteraDiprionidaeNeodiprion fabriciiSawfly
HymenopteraDiprionidaeNeodiprion leconteiRedheaded pine sawfly
HymenopteraDiprionidaeNeodiprion pinetumWhite pine sawfly
HymenopteraDiprionidaeNeodiprion virginianaPine sawfly
HymenopteraEulophidaePhymastichus coffeaParasitoid wasp of coffee berry borer
HymenopteraFormicidaeSolenopsis invictaRed imported fire ant
HymenopteraFormicidaeSolenopsis richteriBlack fire ant
HymenopteraFormicidaeWasmannia auropunctataLitte fire ant
HymenopteraIchneumonidaeVenturia canescensEndoparasitoid
HymenopteraVespidaeVespa crabroEuropean Hornet
HymenopteraVespidaeVespa mandariniaAsian giant hornet
HymenopteraVespidaeVespa mandariniaAsian giant hornet
HymenopteraVespidaeVespa mandariniaAsian giant hornet
HymenopteraVespidaeVespula pensylvanicaWestern yellowjacket
IxodidaArgasidaeOrnithodoros turicataRelapsing fever tick
IxodidaArgasidaeOrnithodoros verrucosusRelapsing fever tick
IxodidaIxodidaeAmblyomma americanumLone star tick
IxodidaIxodidaeAmblyomma maculatumGulf Coast tick
IxodidaIxodidaeAmblyomma variegatumTropical bont tick
IxodidaIxodidaeDermacentor albipictusWinter tick
IxodidaIxodidaeDermacentor andersoniRocky Mountain wood tick
IxodidaIxodidaeDermacentor variabilisAmerican dog tick
IxodidaIxodidaeHaemaphysalis longicornisAsian longhorned tick
IxodidaIxodidaeRhipicephalus appendiculatusBrown ear tick
LepidopteraCrambidaeDiatraea grandiosellaSouthwestern corn borer
LepidopteraCrambidaeOstrinia nubilalisEuropean corn borer
LepidopteraGelechiidaeAnarsia lineatellaPeach twig borer
LepidopteraGelechiidaePectinophora gossypiellaPink bollworm
LepidopteraGelechiidaeSitotroga cerealellaAngoumois grain moth
LepidopteraNoctuidaeEudocima phaloniaFruit piercing moth
LepidopteraNoctuidaeHelicoverpa zeaCorn earworm
LepidopteraNoctuidaeSpodoptera frugiperdaFall armyworm
LepidopteraNoctuidaeSpodoptera frugiperdaFall armyworm
LepidopteraNoctuidaeStriacosta albicostaWestern bean cutworm
LepidopteraPyralidaeAchroia grisellaLesser waxmoth
LepidopteraPyralidaeAmyelois transitellaNavel orangeworm
LepidopteraPyralidaeCadra cautellaAlmond moth
LepidopteraPyralidaeCadra figulilellaRaisin moth
LepidopteraPyralidaePlodia interpunctellaIndian meal moth
LepidopteraSphingidaeManduca sextaTobacco hornworm
LepidopteraTortricidaeCydia pomonellaCodling moth
OrthopteraAcrididaeSchistocerca gregariaDesert locust
OrthopteraGryllidaeAcheta domesticusHouse cricket
OrthopteraGryllidaeGryllodes sigillatusBanded cricket
OrthopteraTettigoniidaeAnabrus simplexMormon cricket
ThysanopteraThripidaeFrankliniella occidentalisWestern flower thrip