Ag100Pest Initiative

The Ag100Pest Initiative is led by the USDA’s Agricultural Research Service. The initiative leverages ARS's unique expertise in both arthropod pest management and agricultural genomics research, and enhances the agency’s contribution to two international genome sequencing projects – i5K (the 5000 insect genomes initiative) and the Earth BioGenome Project (to sequence the genomes of all of the world’s 1.5 million animals and plants).

Goal: Produce annotated, reference quality genome assemblies for the top 100 US arthropod agricultural pests. When possible, generate the PacBio long-read data from a single individual.

Arthropod pests of US field crops, livestock, bees, trees, and stored products as well as foreign pest species considered potential invasive threats to US agriculture are being considered. Beyond genomes, Ag100Pest teams will develop best practices that will benefit the entire arthropod genomics community. We are interested in collaborating with the community in these efforts and hope our successes will encourage others to undertake similar efforts.

Executive Committee: Anna Childers, Brian Scheffler, Kevin Hackett
Core Team: Brad Coates, Scott Geib, Brian Scheffler, Tim Smith, Anna Childers, Monica Poelchau, Chris Childers, Kevin Hackett

The 74 species in the table below are currently included in the effort. This list is not final; additions and modifications will occur as the project progresses.
Last updated: 2019-11-15

OrderFamilyScientific NameCommon NameEstimated Genome Size (Mbp)BioProjectPublished
ColeopteraAnobiidaeLasioderma serricorneCigarette beetle155
ColeopteraAnobiidaeStegobium paniceumDrugstore beetle200
ColeopteraBostrichidaeRhyzopertha dominicaLesser grain borer480 PRJNA449115
ColeopteraCerambycidaeAcalolepta aestheticaQueensland longhorned beetle600
ColeopteraCerambycidaeMallodon dasystomusHardwood stump borer1800
ColeopteraChrysomelidaeChrysomela scriptaCottonwood leaf beetle1200
ColeopteraChrysomelidaeDiabrotica barberiNorthern corn rootworm2500
ColeopteraChrysomelidaeDiabrotica undecimpunctataSouthern corn rootworm1250
ColeopteraChrysomelidaeDiorhabda carinataLarger tamarisk beetle350
ColeopteraChrysomelidaeDiorhabda carinulataNorthern tamarisk beetle350
ColeopteraChrysomelidaeDiorhabda elongataMediterranean tamarisk beetle350
ColeopteraChrysomelidaeDiorhabda sublineataSubtropical tamarisk beetle350
ColeopteraCurculionidaeAnthonomus grandisBoll weevil800
ColeopteraCurculionidaeSitophilus oryzaeRice weevil768
ColeopteraDermestidaeTrogoderma granariumKhapra beetle300
ColeopteraDermestidaeTrogoderma granariumKhapra beetle300
ColeopteraDermestidaeTrogoderma variabileWarehouse beetle275
ColeopteraScarabaeidaeOryctes rhinocerosCoconut rhinoceros beetle550
ColeopteraSilvanidaeOryzaephilus mercatorMerchant grain beetleunknown
ColeopteraSilvanidaeOryzaephilus surinamensisSaw-toothed grain beetle155
ColeopteraTenebrionidaeTenebrio molitorYellow mealworm775
ColeopteraTenebrionidaeTribolium castaneumRed flour beetle205
ColeopteraTenebrionidaeTribolium confusumConfused flour beetle300
ColeopteraTenebrionidaeTribolium destructorDestructive flour beetle170
DipteraMuscidaeHaematobia irritansHorn fly1200
DipteraMuscidaeMusca autumnalisFace fly, female1200
DipteraMuscidaeMusca autumnalisFace fly male1200
DipteraMuscidaeStomoxys calcitransStable Fly1130
DipteraTephritidaeAnastrepha fraterculusTephritid fruit fly pest850
DipteraTephritidaeAnastrepha ludensMexican fruit fly800
DipteraTephritidaeAnastrepha obliquaTephritid fruit fly pest850
DipteraTephritidaeAnastrepha suspensaCaribbean fruit fly1200
DipteraTephritidaeBactrocera albistrigataTephritid fruit fly pest675
DipteraTephritidaeBactrocera correctaTephritid fruit fly pest675
DipteraTephritidaeBactrocera zonataTephritid fruit fly pest675
DipteraUlidiidaeEuxesta stigmatiasCorn silk flyunknown
HemipteraAphididaeSchizaphis graminumGreenbug350
HemipteraFulgoridaeLycorma delicatulaSpotted lanternfly2300PRJNA540533GigaScience
HemipteraGeocoridaeGeocoris pallenWestern big-eyed bug1000
HemipteraMiridaeLygus hesperusLygus bug/Western tarnished plant bug1000
HemipteraMiridaeLygus lineolarisLygus bug/Tarnished plant bug860
HemipteraPentatomidaeBagrada hilarisBagrada bug1300
HemipteraPentatomidaeEuschistus servusBrown stink bug1300
HemipteraPentatomidaeHalyomorpha halysBrown marmorated stink bug1200
HemipteraPentatomidaeMurgantia histrionicaHarlequin bug1300
HemipteraPentatomidaeNezara viridulaSouthern green stink bug1300
HemipteraPentatomidaePiezodorus guildiniiRed-banded stink bug1000
HemipteraPlataspidaeMegacopta cribrariaKudzu bug1300
HemipteraPsyllidaeCacopsylla pyricolaPear psylla480
HemipteraReduviidaeRhodinus prolixusKissing bug707
HemipteraScutelleridaeEurygaster integricepsSunn Pestunknown
HemipteraTriozidaeBactericera cockerelliPotato psyllid480
HemipteraTriozidaeBactericera maculipennisBindweed psyllid480
HemipteraTriozidaeLeuronota fagaraeLime psyllid480
HymenopteraDiprionidaeDiprion similisIntroduced pine sawfly350
HymenopteraDiprionidaeNeodiprion fabriciiSawfly350
HymenopteraDiprionidaeNeodiprion virginianusPine sawfly350
HymenopteraFormicidaeSolenopsis invictaRed imported fire ant450
HymenopteraFormicidaeWasmannia auropunctataLitte fire ant300
HymenopteraVespidaeVespa crabroEuropean Hornetunknown
HymenopteraVespidaeVespula pensylvanicaWestern yellowjacket220
IxodidaArgasidaeOrnithodoros turicataRelapsing fever tick, female1200
IxodidaArgasidaeOrnithodoros verrucosusArgasid tick1200
LepidopteraCrambidaeOstrinia nubilalisEuropean corn borer455PRJNA534504
LepidopteraGelechiidaePectinophora gossypiellaPink bollworm500
LepidopteraNoctuidaeEudocima phaloniaFruit piercing moth600
LepidopteraNoctuidaeHelicoverpa zeaCorn earworm363
LepidopteraNoctuidaeStriacosta albicostaWestern bean cutworm1270
LepidopteraPyralidaeAchroia grisellaLesser waxmothunknown
LepidopteraPyralidaePlodia interpunctellaIndian meal moth325
LepidopteraTortricidaeCydia pomonellaCodling moth650
OrthopteraGryllidaeAcheta domesticusHouse cricket2250
OrthopteraGryllidaeGryllodes sigillatusBanded cricket2000
PhasmatodeaDiapheromeridaeSiploidea sipylusPink winged stick insectunknown